《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年2 页码: 118-124 栏目: 出版日期: 2024-01-31

Title:Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong

文章编号:1001-0009(2024)02-0118-07

作者:彭浩1; 曹丹1; 司豆豆1; 张升明2; 3; 张怀荣4; 李娜1（1.陕西理工大学 生物科学与工程学院，陕西 汉中 723000
；2.J9集团，陕西 汉中 723500
；3.陕西省西乡县食用菌钻研所，陕西 汉中 723500
；4.宁强县真菌钻研所，陕西 汉中 724400）

Author(s):PENG Hao1; CAO Dan1; SI Doudou1; ZHANG Shengming2; 3; ZHANG Huairong4; LI Na1(1.School of Bioscience and Engineering，Shaanxi University of Technology，Hanzhong，Shaanxi 723000
；2.Shaanxi Chunsen Mushroom Industry Co.Ltd.，Hanzhong,Shaanxi 723500
；3.Xixiang County Edible Fungi Research Institute，Hanzhong,Shaanxi 723500;4.Ningqiang County Institute of Fungi，Hanzhong,Shaanxi 724400)

关键词:天麻; 萌发菌; 拮抗; ITS; 酯酶同工酶

Keywords:Gastrodia elata; germination fungi; antagonism; ITS; esterase isozyme

分类号:Q 939

DOI:10.11937/bfyy.20232599

文件标志码:A

提要:以汉中地域9株天麻萌发菌及天麻花粉种子为试材，选取拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等步骤，钻研了9株天麻萌发菌的遗传多样性、亲缘关系和促萌成效，以期为天麻萌发菌的种质资源治理与评价等提供参考凭据
。了局批注：9株萌发菌凭据两两拮抗试验的水平分歧分为不显著、显著和极显著3类
；凭据ITS序列的差距分为Mycena sp.和Heydenia sp. 2类
；凭据酯酶同工酶酶谱条带在80% 的类似水平上可分为5类
；9株萌发菌与天麻种子共生萌发，其中C3菌株菌丝成长速度最高为（0.30±0.01）cm·d-1，C2菌株的萌发率最高为64.66%±2.89%，C2和C3菌株的综合阐发最佳
。

Abstract:The genetic diversity，genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment，ITS sequence determination，esterase isozyme technology and seed mixing，in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types，insignificant，significant and extremely significant according to the degree of antagonism of each pair
；according to the difference of ITS sequence，they were classified into Mycena sp.and Heydenia sp.2
；according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%
；9 germination strains co-germinate with Gastrodia elata seeds，of which the highest growth rate of C3 strain was (0.30±0.01)cm·d-1，and the highest germination rate of C2 strain was 64.66%±2.89%，the comprehensive performance of C2 and C3 strains were the best.